SETA (CIN85, Ruk, SH3KBPI) (HRP), Clone: [5H43 84], Mouse, Monoclonal
Artikelnummer:
USB-S1012-10-HRP
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| Artikelname: | SETA (CIN85, Ruk, SH3KBPI) (HRP), Clone: [5H43 84], Mouse, Monoclonal |
| Artikelnummer: | USB-S1012-10-HRP |
| Hersteller Artikelnummer: | S1012-10-HRP |
| Alternativnummer: | USB-S1012-10-HRP-100 |
| Hersteller: | US Biological |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | IP, WB |
| Immunogen: | 6His and HA tagged fusion protein corresponding to full length rat SETA. |
| SETA (CIN85, Ruk/SH3KBPI) regulates the ligand-dependent endocytosis of the IgE receptor. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml detects SETA in RIPA lysates from NG108-15 cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate SETA in human U87 glioma cells and rat primary astrocytes. Immunocytochemistry: Has been reported by an independent laboratory to detect SETA in rat primary astrocytes. Optimal dilutions to be determined by the researcher. Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody. |
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