Recombinant protein corresponding to mouse SF-20 expressed in E. coli.
Mouse SF20 is a secreted bone marrow stroma-derived growth factor. SF20 isnot only expressed in bone marrow and spleen stroma cells, It is expressed in resting mononuclear cells, resting CD8+ and CD19+ cells and activated CD8+ T cells. It has been shown only to bind to the surface of cells expressing the receptor TSA-1 (Thymic shared Ag-1). Reports show that SF20 stimulates the proliferation of FDCP2 (a mouse IL-3 factor-dependent hemopoietic cell line) cells and mouse lymphoid cells through TSA-1, but it does not show any myelopoietic activity. More research on SF20 and its receptor TSA-1 may help determine the role they play in the regulation of lymphocyte activation. Recombinant Mouse SF20 is a 15.8kD protein, consisting of 143 amino acid residues. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: To detect mSF-20 by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant mSF-20. Western Blot: To detect mSF-20 by Western Blot analysis S1012-83M can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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