SFRP3 (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1) (Biotin), Goat
Artikelnummer:
USB-S1012-88D
Hersteller Artikelnummer:
S1012-88D
Alternativnummer:
USB-S1012-88D-50
Hersteller:
US Biological
Wirt:
Goat
Kategorie:
Antikörper
Applikation:
IHC, WB
Immunogen:
Recombinant protein corresponding to Ala33-Ser323 from mouse SFRP3, expressed in NS0 cells.
SFRP3, also known as FRZB, Fritz, Frzb1 and FRP-3, is expressed in the developing bone and cartilage as well as several adult tissues. It functions as an antagonist of Wnt signaling. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml, The detection limit for recombinant mouse SFRP3 is ~5ng/lane under non-reducing and reducing conditions. In this format, shows ~60% crossreactivity with recombinant human SFRP3. Immunohistochemistry: 2-5ug/ml, Detects SFRP3 in cells and tissues. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
