SFRP4 (Secreted Frizzled Related Protein 4, DDC-4, FrpAP, frpHE, FrzB-2), Goat
Artikelnummer:
USB-S1012-89
Hersteller Artikelnummer:
S1012-89
Alternativnummer:
USB-S1012-89-100
Hersteller:
US Biological
Wirt:
Goat
Kategorie:
Antikörper
Applikation:
ELISA, WB
Immunogen:
Recombinant protein corresponding to Ala22-Val346 from human SFRP4 expressed in NS0 cells.
sFRP4 belongs to the sFRP family whose members contain a cysteine-rich domain homologous to the Wnt-binding domain of the Frizzled proteins. SFRP4 is a circulating protein that can function as a phosphatonin as well as an antagonist of Wnt signaling. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml with OVCAR-3 human ovarian carcinoma cell line, human ovarian cancer tissue, and MCF-7 human breast cancer cell line under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
