Shh (Sonic HedgeHog), IgG2a, Clone: [15j188], Rat, Monoclonal
Artikelnummer:
USB-S1012-96E
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| Artikelname: | Shh (Sonic HedgeHog), IgG2a, Clone: [15j188], Rat, Monoclonal |
| Artikelnummer: | USB-S1012-96E |
| Hersteller Artikelnummer: | S1012-96E |
| Alternativnummer: | USB-S1012-96E-500 |
| Hersteller: | US Biological |
| Wirt: | Rat |
| Kategorie: | Antikörper |
| Applikation: | ELISA, WB |
| Immunogen: | Recombinant protein corresponding to Cys199-Ser437 from mouse Sonic Hedgehog at C-terminal, expressed in E. coli. |
| Members of the Hedgehog (Hh) family are highly conserved proteins which are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). The biologically active form of Hh molecules is obtained by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal one half of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can substitute for each other in experimental systems. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using Recombinant Mouse Sonic Hedgehog Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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