A 17 aa synthetic peptide witin 1st putative extracellualr domain (between 1-2 TM) of human silain was synthesized, conjugated to KLH
Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique. Sialin is ~55-60kD protein. ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Affinity purified over the control-peptide Sepharose
Formulierung:
Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
