A 17 aa synthetic peptide witin 1st putative extracellualr domain (between 1-2 TM) of human silain was synthesized, conjugated to KLH
Sialic acid storage disease (SASD) are autosomal recessive neurdegenerative disorders that may present as a severa infantile form (ISSD) or a slowly progressive adult form (Salla disease) prevalent in Finland. The patients exccrete large amounts of free sialic acid in urine. A H+/anionic sugar symporter mechanism for sialinc acid and glucoronic acid is impaired in lysosomal membranes from Sall and ISSD. A new gene, termed Sialin (SLC17A5, human 495 aa, chromosome 6q14-q15) belonging to the family of anion/cation symporters (ACS) has been found to be mutated in sialic acid storage disease. Sialin is predicted to contain up to 12 TM domains with N and C-termini located in the cytoplasm. Sialin has 37%, 34%, and 16% sequence identity with BNPI/VGLUT1, NPT1 (Napi-I), and E. coli hexuronate (EcHex) transporter, respectively. Sialin is found to be expressed in many human tissues. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution:. Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique. Sialin is ~55-60kD protein. ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommeded for ELISA, dot blot, or antibody blocking experiments (use 5-10ug of control peptide per 1ug of affinity purified IgG or 1ul of antiserum) to demonstrate antibody specificity. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Serum
Formulierung:
Supplied as a liquid, neat serum, 40% glycerol.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
