Siglec-1 cell surface antigen, also known as CD169, is expressed by subsets of cells of the macrophage/ monocyte cell lineage. CD169 is preferentially expressed on cells in the late stages of maturation. It is expressed strongly on alveolar and tissue macrophages, but weakly on blood monocytes. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Westen Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10-1:100, 10ul labels 10e6 cells in 100ul. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Positive Control for Immunohistochemistry: Porcine spleen Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
