Siglec-1 cell surface antigen, also known as CD169, is expressed by subsets of cells of the macrophage/ monocyte cell lineage. CD169 is preferentially expressed on cells in the late stages of maturation. It is expressed strongly on alveolar and tissue macrophages, but weakly on blood monocytes. Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation and Westen Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Positive Control for Immunohistochemistry: Porcine spleen Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
