Synthetic peptide corresponding to 21aa of human Survivin (Accession O15392-2) (KLH).
Apoptosis is a form of cell death that permits the removal of damaged, senescent or unwanted cells in multicellular organisms, without damage to the cellular microenvironment. Survivin is a member of the inhibitor of apoptosis (IAP) gene family, which encodes negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. Survivin appears to have an important role in regulating apoptosis at cell cycle checkpoint(s). Survivin expression is highly cell cycle-regulated, and is detectable in the nucleus selectively at the G2/M phase. Survivin (isoform 2, Beta): Human: 165aa, 18.6kD, chromosome: 17q25, mainly expressed in fetal kidney and liver, and to lesser extent, lung and brain, abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml (ECL) ELISA: 1:100,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
