Synthetic peptide corresponding to 12aa near the N-terminus of human Survivin. Species sequence homology: porcine and bovine (82%), rat (58%)
Survivin also known as IAP (Inhibitor of Apoptosis) or TIAP (murine homologue of IAP) is undetectable in quiescent tissue but abundantly expressed in proliferating cells and it has widespread tissue distribution. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. All the iap genes isolated from different species have a common motif known as baculovirus IAP repeat (BIR) which may be present in two or three copies. The BIR motifs are essential for interaction of the IAP proteins with proapoptotic proteins, including the caspase family of death proteases. Survivin has been associated with protection against apoptosis of cells that exit mitosis abberantly. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction which is regulated by microtubule dynamics and rapidly downregulated by cell cycle arrest in G1 phase. (2,3). Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested. Recommended Diltuions: Western Blot: 1-5ug/ml Immunocytochemistry: 5ug/ml Immunofluorescence: 20ug/ul Immunohistochemistry (paraffin): 5ug/ml Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
