Synthetic peptide corresponding to the sequence near the N-terminus of rat Syntaxin 2.
Syntaxin 2, also known as epimorphin, is a 35kD type II integral membrane that belongs to the t-SNARE family, a group of proteins involved in protein transport (2). Syntaxin 2 was first identified as a factor that promotes branching morphogenesis in mammary epithelial cells (3). Studies using pancreatic exocrine acinar cells, in which the exocytic pathways are well defined, show primary localization of syntaxin 2 to the apical plasma membrane (4). Homologs of syntaxin 2 have been identified in various eukaryotes ranging from yeast to human (5, 6). Neuronal syntaxin is a component of a 20S complex implicated in vesicle docking and fusion, which involves its interaction with syntaxin through a ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP, and NSF (7). Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 10ug/ml Immunohistochemistry: paraffin Western Blot (colormetric): 1ug/ml Optimal dilutions to be determined by the researcher. Positive Controls: Jurkat Cell Lysate HeLa Cell Lysate Rat Brain Tissue Extract Mouse Brain Tissue Extract Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
