Recombinant human Syntaxin 16 isoform B aa165-301, O14662 (E. Coli).
Syntaxin 16 (STX16, also SNY16) is a 39kD member of the syntaxin family of proteins. It is ubiquitously expressed, embedded in the Golgi membrane, and participates in the fusion of early endosomes with the Golgi stacks. Syntaxin 16 contributes one of four coiled-coil domains necessary for retrograde transport. Human Syntaxin 16 is a type IV single-pass transmembrane protein (very short lumenal C-terminus) that is 325aa in length. It contains a cytoplasmic syntaxin region (aa74-180), a coiled-coil region (aa230-292), and a short, three amino acid C-terminal lumenal sequence. There are multiple potential isoforms. Two show alternate start sites at Met187 and Met54, while three others show deletions of aa45-48, 28-48 and 28-44, respectively. A cytoplasmic form shows an Ala substitution for aa 132-325. Over aa165-301, human Syntaxin 16 shares 95% aa identity with mouse Syntaxin 16. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
