Recombinant full-length nitrated/oxidized human alpha-Synuclein. Species Sequence Homology: mouse and rat: 95%.
Alp[ha-synuclein belongs to the synuclein family, which also includes b- and g-synuclein, and is predominantly expressed in neurons, concentrated at synaptic terminals. Synucleins may be involved in the regulation of dopamine release and transport and the modulation of synaptic vesicle function. a-synuclein is a small, presynaptic protein that is the major non-amyloid component of the pathological inclusions characteristic of a wide range of neurodegenerative disorders, collectively known as synucleinopathies. Synucleinopathies include Parkinsons disease (PD), Lewy body variant of Alzheimers disease (LBVAD), dementia with Lewy bodies (DLB), neurodegeneration with brain iron accumulation (NBIA-1), and multiple system atrophy (MSA). Although alpha-synuclein is a soluble protein, it polymerizes into filaments in vitro under a variety of conditions, lending more support to the finding that it is the major building block of the filaments that form Lewy bodies (LBs). alpha-synuclein is known to reduce the fibrillization of the microtubule-associated protein, tau, and to bind various targets such as 14-3-3, protein kinase C, synphilin-1, Elk, and Tat-binding protein Applications: Suitable for use in Immunofluorescence, FLISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
