TAB1 (TGF-beta-activated Kinase 1 and MAP3K7-binding Protein 1, TGF-beta-activated Kinase 1-binding Protein 1, TAK1-binding Protein 1, 3-Tab1, Mitogen-activated Protein Kinase Kinase Kinase 7-interacting Protein 1, MAP3K7IP1), Clo
TAB1 (TGF-beta-activated Kinase 1 and MAP3K7-binding Protein 1, TGF-beta-activated Kinase 1-binding Protein 1, TAK1-binding Protein 1, 3-Tab1, Mitogen-activated Protein Kinase Kinase Kinase 7-interacting Protein 1, MAP3K7IP1), Clo
TAB1 (TGF-beta-activated Kinase 1 and MAP3K7-binding Protein 1, TGF-beta-activated Kinase 1-binding Protein 1, TAK1-binding Protein 1, 3-Tab1, Mitogen-activated Protein Kinase Kinase Kinase 7-interacting Protein 1, MAP3K7IP1), Clo
Artikelnummer:
USB-T0291-85
Hersteller Artikelnummer:
T0291-85
Alternativnummer:
USB-T0291-85-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IP, WB
Immunogen:
Synthetic peptide corresponding to residues surrounding Pro381 of human TAB1.
TAK1 is a mitogen-activated protein kinase kinase kinase activated by TGF-beta and various pro-inflammatory signals (1,2). In vivo TAK1 activation requires its association with TAK1 binding protein 1 (TAB1), which triggers TAK1 autophosphorylation at Thr184 and Thr187 (3,4). The TAB2 adaptor protein links TAK1 with TRAF6 to mediate TAK1 activation following IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK respectively. TAK1 and TRAF6 also activate the NF-kB pathway by phosphorylating the NF-kB inducing kinase (NIK) to trigger subsequent activation of IKK (2,6). In addition to TAK1, TAB1 interacts with and activates p38alpha (7). Targeted disruption of the TAB1 gene in mice causes a drastic reduction in TAK1 activity and leads to embryonic lethality (8). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
