Recombinant protein corresponding to Ser24-Ala618 from mouse Tamm-Horsfall Glycoprotein, expressed in NS0 cells.
Tamm-Horsfall glycoprotein (THP, Uromodulin) is a 105-120kD urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL-1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619aa in length. It contains three EGF-like domains (aa28-148), a ZP domain that mediates oligomerization (aa335-590), and a cleavable C-terminal propeptide (aa619- 642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17aa substitution for aa601-642, and a 166aa substitution for aa441- 642. Over aa25-614, mouse Uromodulin shares 78% and 89%aa identical to human and rat Uromodulin, respectively. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using mouse kidney tissue Immunohistochemistry: 5-15ug/ml using immersion fixed frozen sections of mouse kidney Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
