Recombinant protein corresponding to Ser24-Ala618 of mouse Uromodulin expressed in NSO cells. Species Sequence Homology: rat, 89%, human: 78 over aa25-614.
Uromodulin (also Tamm-Horsfall glycoprotein or THP) is a 105-120kD urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL-1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619 amino acids (aa) in length. It contains three EGF-like domains (aa28-148), a ZP domain that mediates oligomerization (aa335-590), and a cleavable C-terminal propeptide (aa619-642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17aa substitution for aa601-642, and a 166 aa substitution for aa441-642. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant mouse Uromodulin is ~1ng/lane and 20ng/lane under non-reducing and reducing conditions, respectively. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile TBS, pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from PBS, BSA (50ug per ug antibody). Labeled with Biotin. Reconstitute with 1ml sterile TBS, (20mM Trizma base, 150mM sodium chloride) pH 7.3, 0.1% BSA.
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