A 17-aa Peptide sequence within the N-terminal (after the 1st poly-pro) domain of human TANK1 (1) was synthesized, coupled to KLH
The 3ends of chromosomes are capped with telomere sequences (TTAGGG, 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. Introduction of telomerase into normal human cells has been shown to extend normal cell life by ~ 20 doubling. Poly(ADP-ribose) polymerases (PARPs) catalyze formation of long, branched chain of poly(ADP-ribose) onto protein acceptors using NAD+ as a substrate. Poly(ADP)ribosylation is a transient posttranslational modification that can either enhance or reduce protein activity. Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase, human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere. TANK1 is alternatively spliced to isoform 1 and 2 (missing 644-1327). The N-terminal HPS domain contains multiple run of histidine, proline, and serine residue homopolymers. TANK1 has 24 ankyrin repeats in TRF-1 interacting domain near the N-terminus. The 33-aa ANK repeat motif mediates protein-protein interactions. The ANK domain is followed another protein interaction motif called the sterile alpha-module (SAM). The C-terminal region of TANK1 contains the PARP activity. TANK1 uses its ANK domain to bind TRF1 and its PARP domain to ADP-ribosylate itself and TRF1, and thereby inhibiting the ability of TRF1 to bind telomere. The homology between tankyrase and PARPs is limited to catalytic domain. Takyrase-1 is expressed in many tissues and targeted to various intracellular compartments. Tanyrase-1, devoid of NLS (nuclear localization signal), is translocated to telomere (nucleus) through binding of its ANK domain to TRF1. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml using ECL. ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA and 0.05% sodium azide, before the addition of glycerol to 40%.
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