Synthetic peptide corresponding to the 14-aa between the ANK15 and SAM domain of human TANK2 (KLH).
The 3ends of chromosomes are capped with telomere sequences (TTAGGG, 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. Introduction of telomerase into normal human cells has been shown to extend normal cell life by ~ 20 doubling. Poly(ADP-ribose) polymerases (PARPs) catalyze formation of long, branched chain of poly(ADP-ribose) onto protein acceptors using NAD+ as a substrate. Poly(ADP)ribosylation is a transient posttranslational modification that can either enhance or reduce protein activity. Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase, human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere. The N-terminal HPS domain contains multiple run of histidine, proline, and serine residue homopolymers. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml. Using ECL. TANK1 ~130kD. ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Control Peptide: T0900-08: Tankyrase, Human (TRF1 interacting ankyrin-related ADP-ribose polymerase, TANK2, TNKL) Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
