Synthetic phosphopeptide derived from a region of human tau that contains serine 404. The sequence is conserved in mouse and rat.
Tau is a neuronal microtubule-associated protein found predominantly on axons that functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimers disease (AD) brain. Hyperphosphorylated tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II, phosphorylate tau. Serine 404 is phosphorylated by GSK-3beta and cdk5 in vitro and in vivo Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot 1:1000 Optimal dilutions to be determined by the researcher. Positive Control: Recombinant human tau treated with GSK-3beta. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.2. No stabilizing proteins added. No preservative added. Glycerol-free. Also available wth azide, BSA, glycerol. See T1032-70.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
