recombinant human alpha-Taxilin, aa1-162, P40222 (E. coli).
Alpha-Taxilin (TXLN-alpha, also p75 [in rat] and IL-14 alpha) is the founding member of the taxilin family of proteins. Although its predicted MW is 62kD, it runs anomalously at 74-76kD in SDS-PAGE. Alpha-Taxilin is ubiquitously expressed and participates in intracellular vesicle trafficking. In particular, alpha-Taxilin interacts with syntaxin family members that localize to the plasma membrane, providing for SNARE complex formation. alpha-Taxilin also regulates the placement of growing protein chains. Human alpha-Taxilin is 546aa in length. Its defining characteristic is a large coiled-coil region between aa186-491. When an alternate start site at Met176 is used, a secreted cytokine is generated termed IL-14 alpha. Over aa1-162, human alpha-Taxilin shares 81% aa identity with mouse alpha-Taxilin. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
