Synthetic peptide corresponding to SASDGSVCVLDLRK from the C-terminus from human TBL1 (NP_005638.1, NP_078941.2). Species sequence homology: mouse, rat, canine and bovine
Transducin beta-like protein 1 (TBL1) is part of an SMRT-containing complex. Mutation of the TBL1 gene is linked with human sensorineural deafness. TBL1 displays structural and functional similarities to Tup1 and Groucho corepressors, sharing their ability to interact with histone H3. Applications: Suitable for use in Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 4-6ug/ml, in paraffin embedded Human Kidney shows preferential nuclear staining in the DCT Peptide ELISA: 1:4000 Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See T1510-01-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
