Synthetic peptide corresponding to residues surrounding Glu81 of human TCF4. Species Sequence Homology: mouse, rat
LEF1/TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors which consists of four members: Lymphoid enhancer factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3 and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and are downstream factors in the Wnt signaling pathway. LEF1/TCF bind to Wnt response elements to provide a docking site for beta-catenin which translocates to the nucleus and promotes the transcription of target genes upon activation of Wnt signaling (3). LEF1/TCF proteins are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers such as colon cancers (4,5). TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting studies indicate that TCF4 is required to maintain the crypt stem cells of the small intestine (6,7). TCF4 has several slicing isoforms which are expressed differentially in tissues and during cancer progression (8,9). Studies also indicate that a variant of the TCF4 gene confers an increased risk of type 2 diabetes (10). Applications: Suitable for use in Western Blot, Immunoprecipitation and Chromatin Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4C with gentle shaking, overnight. Immunoprecipitation: 1:50 Chromatin Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
