T-Complex Protein 1 alpha (TCP-1a, CCT), IgG2c, Clone: [4E215], Rat, Monoclonal
Artikelnummer:
USB-T2035-01
Hersteller Artikelnummer:
T2035-01
Alternativnummer:
USB-T2035-01-50,USB-T2035-01-200
Hersteller:
US Biological
Wirt:
Rat
Kategorie:
Antikörper
Applikation:
IHC, IP, WB
Immunogen:
Recombinant protein corresponding to mouse TCP-1alpha (C-terminal fragment)
TCP-1alpha is a 60kD subunit of a cytosolic hetero-oligomer chaperone (1,2) that is known to be involved in the folding of actin and tubulin. This protein is a member of the chaperonin family, which includes Escherichia coli GroEL, the mitochondrial heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein and the archaebacterial protein TF55. These chaperonins assist the folding of proteins upon ATP hydrolysis. Nine different subunits of TCP-1 containing chaperonin complexes from mammalian testis and seven different subunits of mouse F9 cells have been identified. TCP-1 has also been referred to as eTRici, TCP-1 ring complex or the chaperonin containing complex, CCT. The COOH group is probably recognized since addition of an alanine (LDDACOOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences. T2035-01 binds Protein A and is useful for native precipitations of coatomer and CTT. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot (Colormetric): 1:1000 Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
