T-Complex Protein 1 alpha (TCP-1a, CCT), IgG2a, Clone: [91a], Rat, Monoclonal
Artikelnummer:
USB-T2035-02
Hersteller Artikelnummer:
T2035-02
Alternativnummer:
USB-T2035-02-50,USB-T2035-02-200
Hersteller:
US Biological
Wirt:
Rat
Kategorie:
Antikörper
Applikation:
FC, IHC, IP, WB
Immunogen:
Recombinant protein corresponding to mouse TCP-1alpha (C-terminal fragment).
TCP-1alpha is a 60kD subunit of a cytosolic hetero-oligomer chaperone (1,4) that is known to be involved in the folding of actin and tubulin. This protein is a member of the chaperonin family, which includes Escherichia coli GroEL, the mitochondrial heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein and the archaebacterial protein TF55. These chaperonins assist the folding of proteins upon ATP hydrolysis. Nine different subunits of TCP-1 containing chaperonin complexes from mammalian testis and seven different subunits of mouse F9 cells have been identified. TCP-1 has also been referred to as eTRici, TCP-1 ring complex or the chaperonin containing complex, CCT. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot (colormetric): 1:1000 Flow Cytometry: 1:100 Optimal dilutions to be determined by researcher. Positive Controls: HeLa Heat Shocked Cell Lysate Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
