E. coli-derived recombinant protein corresponding to Met1-Lys180 of human TFCP2L1.
TFCP2L1 (Transcription factor CP2-like protein 1, also LBP-9) is a 54kD (predicted) member of the CP2 subfamily, grh/CP2 family of proteins. It is expressed in placental synctiotrophoblast cells, and regulates the production of CYP11A1/P450scc. P450scc is a cholesterol cleavage enzyme that generates pregnenolone, a necessary intermediate in the formation of progesterone. TFCP2L1 both positively and negatively regulates P450scc synthesis through homodimerization and heterodimerization with LBP-1b. Human TFCP2L1 is 479 amino acids (aa) in length. It contains a transcriptional repressor region (aa100-200) and a dimerization domain (aa300-479). There is one splice variant that shows a deletion of aa400-464. Human TFCP2L1 is 73% aa identical to human TFCP2, and 93% aa identical to mouse CRTR-1, a closely related molecule that may not represent the actual mouse ortholog to TFCP2L1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using human placental tissue Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
