Recombinant corresponding to human TSH R expressed in NSO cells (P16473).
Thyroid stimulating hormone receptor is an 85kD (unglycosylated) member of the G-protein coupled receptor 1 family. Human TSH R is synthesized as a 764aa precursor that contains a 20aa signal sequence, a 393aa extracellular domain (ECD), a 269aa membrane spanning domain consisting of seven transmembrane segments, and an 82aa cytoplasmic domain. The ECD contains six leucine-rich repeats (LRR) and six potential sites of N-linked glycosylation. There are two isoforms, long and short, produced by a splicing variant corresponding to aa253-764 in the long isoform that is missing in the short isoform. Human TSH R is 86-87aa identical to mouse and rat TSH R. TSH R is the receptor for thyroid stimulating hormone, which plays a central role in controlling thyroid cell metabolism. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
