Recombinant protein corresponding to human TIMP-1. Cellular Localization: Cytoplasmic.
Tissue inhibitor of metalloproteinases-1 (TIMP-1) and Tissue inhibitor of metalloproteinases-2 (TIMP-2) are inhibitory enzymes of the matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72kD Type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms 92kD Type IV collagenase (also known as MMP-9 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 1-2ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues is improved by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: Conditioned, serum-free medium from (TPA-treated) human fetal lung cell line HFL-1. Bladder, breast and ovarian carcinoma. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[3F372]
Isotyp:
IgG1
Reinheit:
Purified by Protein G affinity chromatography from ascites.
Formulierung:
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T5580-10BX.
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