Tissue Inhibitor of Metalloproteinases 2 (TIMP2), Clone: [3F373], Mouse, Monoclonal
Artikelnummer:
USB-T5585-10A
Hersteller Artikelnummer:
T5585-10A
Alternativnummer:
USB-T5585-10A-500
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
IHC, WB
Immunogen:
Synthetic peptide from the N-terminal region of human TIMP-2 protein. Cellular Localization: Cytoplasmic MW of Antigen: 21kD Epitope: N-terminal
TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72kD Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kD Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. Applications: Suitable for use in Western Blot and Immunohistochemistry. Not suitable for Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin only): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution determined by the researcher. Positive Control: Conditioned, serum-free medium from (TPA-treated) HFL-1 cells. Pancreatic or colon carcinoma. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[3F373]
Reinheit:
Purified by Protein A affinity chromatography.
Formulierung:
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
