Synthetic peptide mapping to aa55-85 from human TLR3.
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). TLR3 cDNA codes for a protein with approximate molecular weight of 120kD (4). TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) (4-6). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes (6). The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunofluorescence/Immunocytochemistry. Other applications have not been tested. Recommended Dilution: Flow Cytometry (Intercellular): 1ul/1x10e6 cells Immunofluorescence (IC): 1:10-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[4H271 40C1285]
Isotyp:
IgG1
Reinheit:
Purified by Protein G affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, 0.02% sodium azide. Labeled with R-Phycoerythrin (PE).
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