Recombinant protein corresponding to aa238-609 of human Toll-like Receptor 4 expressed in E. coli.
The Toll-like receptor (TLR) family is comprised of Type I transmembrane proteins characterized by an extracellular leucine-rich domain and a cytoplasmic tail that contains a conserved region called the Toll/IL-1 (TIR) domain. First discovered in Drosophila, TLRs recognize specific molecular patterns that are present in microbial components and respond to these components in order to eliminate or limit invading microbes. To date, ten mammalian homologs of TLRs have been described. TLR4 is involved in the recognition of lipopolysaccharide (LPS), an abundant glycolipid of Gram-negative bacteria. Through its association with MD-2 and CD14, TLR4 recognizes and initiates multiple intracellular signaling events such as the activation of NF-kappaB leading to the ultimate synthesis and release of a number of proinflammatory mediators including interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-a). TLR4 has also been associated in the recognition of lipocheic acid (LTA), Hsp60 and Hsp70. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: ELISA: 1:2000-1:4000 Flow Cytometry: 3ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[5K155]
Isotyp:
IgG2a
Reinheit:
Purified
Formulierung:
Supplied as a liquid in borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
