TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 mediates the innate immune response to bacterial lipopolysaccharide. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat. Use 10ul to label 10e6 cells or 100ul whole blood. Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
