Urate Transporter 1 (URAT1) (Not for Export EU), Rabbit
Artikelnummer:
USB-U2005-01
Hersteller Artikelnummer:
U2005-01
Alternativnummer:
USB-U2005-01-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ELISA
Immunogen:
Synthetic 11-aa peptide sequence near the cytoplasmic, C-terminus of human URAT1 ( KLH coupled).
Urate, a naturally occurring product of purine metabolism, is a scavenger of oxidants. Urate exists primarily as a weak acid (pKa ~5.75). Urate concentration is quite high (~200-500 uM) in human due to loss of uricase gene and the presence of an effective reabsorption system. Recently an OAT-related transporter, URAT1, has been identified in the kidney that acts as urate-anion exchanger and effectively regulates urate levels. URAT1/RST/SLC22A12 (mouse 553-aa, human 555-aa, chromosome 11q13, ~60 kD) is ~42% identical with OAT4. Like OAT4, it is predicted to display 12-TM domains with cytoplasmic N and C-terminus. URTA1 is prominent is epithelial cells of proximal tubule of the renal cortex. Patients with idiopathic renal hypouricemia (lack of blood uric acid) have defects in URAT1 gene. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 using ECL technique ELISA: 1:10,000-50,000. Coat ELISA plate with 1ug/ml control peptide. Optimal dilutions to be determined by the researcher. Control Peptide: U2005 Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Serum
Formulierung:
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
