CD31/PECAM1 Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A0378
- Images (7)
| Article Name: | CD31/PECAM1 Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A0378 |
| Supplier Catalog Number: | A0378 |
| Alternative Catalog Number: | ABB-A0378-20UL,ABB-A0378-100UL,ABB-A0378-500UL,ABB-A0378-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | CD31, PECA1, GPIIA, PECAM-1, endoCAM, CD31/EndoCAM |
| The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. |
| Application Dilute: | WB,1:500 - 1:5000|IF-P,1:50 - 1:200|IHC-P,1:50 - 1:200 |
| Application Notes: | Cross-Reactivity: Human,Mouse. ResearchArea: Cancer,Tumor immunology,Invasion and Metastasis,Signal Transduction,Cell Biology Developmental Biology,Cell Adhesion,Cytoskeleton,Immunology Inflammation,CDs,Stem Cells,Hematopoietic Progenitors,Mesenchymal Stem Cells,Cardiovascular,Angiogenesis,Blood. Shipping: Ice Bag |
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Western blot analysis of various lysates using CD31/PECAM1 Rabbit pAb (A0378) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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Western blot analysis of lysates from U-937 cells, using CD31/PECAM1 Rabbit pAb (A0378) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
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Western blot analysis of lysates from THP-1 cells, using CD31/PECAM1 Rabbit pAb (A0378) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD31/PECAM1 Rabbit pAb (A0378) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human placenta using CD31/PECAM1 Rabbit pAb (A0378) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunofluorescence analysis of Human placenta tissue using CD31/PECAM1 Rabbit pAb (A0378) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. |
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Immunofluorescence analysis of Human placenta tissue using CD31/PECAM1 Rabbit pAb (A0378) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. |
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