KIRREL Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A14283
Article Name: KIRREL Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A14283
Supplier Catalog Number: A14283
Alternative Catalog Number: ABB-A14283-20UL,ABB-A14283-100UL,ABB-A14283-1000UL,ABB-A14283-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: NEPH1, KIRREL, NPHS23
NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2, MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).
Clonality: Polyclonal
Molecular Weight: 84kDa
NCBI: 55243
UniProt: Q96J84
Purity: Affinity purification
Sequence: QGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTC
Target: KIRREL1
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Mouse,Rat. ResearchArea: Signal Transduction,Kinase,Tyrosine kinases,Cell Biology Developmental Biology. Shipping: Ice Bag
Western blot analysis of various lysates using KIRREL Rabbit pAb (A14283) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.