RNF181 Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A14405
Article Name: RNF181 Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A14405
Supplier Catalog Number: A14405
Alternative Catalog Number: ABB-A14405-20UL,ABB-A14405-100UL,ABB-A14405-1000UL,ABB-A14405-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: HSPC238, RNF181
RNF181 binds the integrin alpha-IIb (ITGA2B, MIM 607759)/beta-3 (ITGB3, MIM 173470) complex and has E3 ubiquitin ligase activity (Brophy et al., 2008 [PubMed 18331836]).
Clonality: Polyclonal
Molecular Weight: 18kDa
NCBI: 51255
UniProt: Q9P0P0
Purity: Affinity purification
Sequence: MASYFDEHDCEPSDPEQETRTNMLLELARSLFNRMDFEDLGLVVDWDHHLPPPAAKTVVENLPRTVIRGSQAELKCPVCLLEFEEEETAIEMPCHHLFHSSCILPWLSKTNSCPLCRYELPTDDDTYEEHRRDKARKQQQQHRLENLHGAMYT
Target: RNF181
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cell Biology Developmental Biology,Ubiquitin. Shipping: Ice Bag
Western blot analysis of various lysates using RNF181 Rabbit pAb (A14405) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.