RNF125 Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A15165
Article Name: RNF125 Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A15165
Supplier Catalog Number: A15165
Alternative Catalog Number: ABB-A15165-20UL,ABB-A15165-100UL,ABB-A15165-1000UL,ABB-A15165-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: TNORS, TRAC1, TRAC-1, RNF125
This gene encodes a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, this protein associates with membranes and is primarily localized to intracellular membrane systems. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway.
Clonality: Polyclonal
Molecular Weight: 26kDa
NCBI: 54941
UniProt: Q96EQ8
Purity: Affinity purification
Sequence: LEVLHQPVRTRCGHVFCRSCIATSLKNNKWTCPYCRAYLPSEGVPATDVAKRMKSEYKNCAECDTLVCLSEMRAHIRTCQKYIDKYGPLQELEETAARCVCPFCQRELYEDSLLDHCITHHRSERRPVFCPLCRLIPDENPSSFSGSLIRHLQVSHTLFY
Target: RNF125
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,Cell Biology Developmental Biology,Cell Cycle,Ubiquitin. Shipping: Ice Bag
Western blot analysis of various lysates, using RNF125 Rabbit pAb (A15165) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.