ANG Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A1546
- Images (1)
| Article Name: | ANG Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A1546 |
| Supplier Catalog Number: | A1546 |
| Alternative Catalog Number: | ABB-A1546-100UL,ABB-A1546-20UL,ABB-A1546-500UL,ABB-A1546-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | ALS9, RAA1, HEL168, RNASE4, RNASE5, ANG |
| The protein encoded by this gene is a member of the RNase A superfamily though it has relatively weak ribonucleolytic activity. This protein is a potent mediator of new blood vessel formation and thus, in addition to the name RNase5, is commonly called angiogenin. This protein induces angiogenesis after binding to actin on the surface of endothelial cells. This protein also accumulates at the nucleolus where it stimulates ribosomal transcription. Under stress conditions this protein translocates to the cytosol where it hydrolyzes cellular tRNAs and influences protein synthesis. A signal peptide is cleaved from the precursor protein to produce a mature protein which contains a nuclear localization signal, a cell binding motif, and a catalytic domain. This protein has been shown to be both neurotrophic and neuroprotective and the mature protein has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Due to its effect on rRNA production and angiogenesis this gene plays important roles in cell growth and tumor progression. Mutations in this gene are associated with progression of amyotrophic lateral sclerosis (ALS). This gene and the neighboring RNase4 gene share promoters and 5 exons though each gene then splices to a distinct 3 exon containing the complete coding region of each gene. Alternative splicing results in multiple transcript variants encoding the same protein. |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Rat. ResearchArea: Epigenetics Nuclear Signaling,Cancer,Invasion and Metastasis,Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Microfilaments,Growth factors,Endocrine Metabolism,Nucleotide metabolism,Cardiovascular,Angiogenesis,Hypoxia. Shipping: Ice Bag |
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Western blot analysis of various lysates, using ANG Rabbit pAb (A1546) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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