PUS10 Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A15962
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| Article Name: | PUS10 Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A15962 |
| Supplier Catalog Number: | A15962 |
| Alternative Catalog Number: | ABB-A15962-100UL,ABB-A15962-20UL,ABB-A15962-500UL,ABB-A15962-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | DOBI, Hup10, CCDC139, PUS10 |
| Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]). |
| Clonality: | Polyclonal |
| Molecular Weight: | 60kDa |
| NCBI: | 150962 |
| UniProt: | Q3MIT2 |
| Purity: | Affinity purification |
| Sequence: | VAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD |
| Target: | PUS10 |
| Antibody Type: | Primary Antibody |
| Application Dilute: | WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Apoptosis,Caspases,Endocrine Metabolism,Mitochondrial metabolism. Shipping: Ice Bag |
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Western blot analysis of various lysates using PUS10 Rabbit pAb (A15962) at 1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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