alpha-Actinin-4 Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A16310
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| Article Name: | alpha-Actinin-4 Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A16310 |
| Supplier Catalog Number: | A16310 |
| Alternative Catalog Number: | ABB-A16310-100UL,ABB-A16310-20UL,ABB-A16310-500UL,ABB-A16310-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | FSGS, FSGS1, ACTININ-4, alpha-Actinin-4 |
| Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. |
| Application Dilute: | WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Cancer,Invasion and Metastasis,Signal Transduction,Cell Biology Developmental Biology,Cell Adhesion,Cytoskeleton,Microfilaments,Extracellular Matrix,Endocrine Metabolism,Immunology Inflammation,T Cell Receptor Signaling Pathway. Shipping: Ice Bag |
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Western blot analysis of various lysates using alpha-Actinin-4 Rabbit pAb (A16310) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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