NGEF Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A16507
Article Name: NGEF Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A16507
Supplier Catalog Number: A16507
Alternative Catalog Number: ABB-A16507-100UL,ABB-A16507-20UL,ABB-A16507-1000UL,ABB-A16507-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: EPHEXIN, ARHGEF27, NGEF
Enables guanyl-nucleotide exchange factor activity. Predicted to be involved in several processes, including activation of GTPase activity, ephrin receptor signaling pathway, and negative regulation of dendritic spine morphogenesis. Predicted to be located in cytosol. Predicted to be active in glutamatergic synapse.
Clonality: Polyclonal
Molecular Weight: 82kDa
NCBI: 25791
UniProt: Q8N5V2
Purity: Affinity purification
Sequence: RVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKE
Target: NGEF
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:2000|IF/ICC,1:50 - 1:100|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,G protein signaling,Small G proteins,Neuroscience. Shipping: Ice Bag
Western blot analysis of various lysates using NGEF Rabbit pAb (A16507) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.