FOS Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A17351
Article Name: FOS Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A17351
Supplier Catalog Number: A17351
Alternative Catalog Number: ABB-A17351-20UL,ABB-A17351-100UL,ABB-A17351-1000UL,ABB-A17351-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: p55, AP-1, C-FOS, FOS
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Clonality: Polyclonal
Molecular Weight: 41kDa
NCBI: 2353
UniProt: P01100
Purity: Affinity purification
Sequence: MMFSGFNADYEASSSRCSSASPAGDSLSYYHSPADSFSSMGSPVNAQDFCTDLAVSSANFIPTVTAISTSPDLQWLVQPALVSSVAPSQTRAPHPFGVPA
Target: FOS
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Tumor biomarkers,Signal Transduction,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Immunology Inflammation,T Cell Receptor Signaling Pathway,Neuroscience, Cell Type Marker,Neuron marker. Shipping: Ice Bag
Western blot analysis of various lysates using FOS Rabbit pAb (A17351) at 1:1000 dilution. NIH/3T3 cells were treated with PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.