NUF2 Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A17553
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| Article Name: | NUF2 Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A17553 |
| Supplier Catalog Number: | A17553 |
| Alternative Catalog Number: | ABB-A17553-100UL,ABB-A17553-20UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | CDCA1, CT106, NUF2R, NUF2 |
| This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. |
| Application Dilute: | WB,1:100- 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Cell Biology Developmental Biology,Cell Cycle. Shipping: Ice Bag |
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Western blot analysis of various lysates using NUF2 Rabbit mAb (A17553) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
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