Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Alternative Names:
PYM, WIBG, PYM1
Enables ribosome binding activity. Involved in exon-exon junction complex disassembly, nuclear-transcribed mRNA catabolic process, nonsense-mediated decay, and positive regulation of translation. Located in cell junction, cytosol, and nuclear lumen. Part of exon-exon junction complex.
WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:
Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling. Shipping: Ice Bag
Western blot analysis of various lysates using PYM1 Rabbit pAb (A18669) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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