It is well know now that biotin plays important roles in a variety of critical metabolic reactions in the cell, and thus, is essential for normal human health, growth and development. Biotin is a water-soluble vitamin required by all organisms by virtue of its essential role in carboxylation reactions. Streptavidin and its homologs (together referred to as streptavidin) are widely used in molecular science owing to their highly selective and stable interaction with biotin.
Clonality:
Polyclonal
Concentration:
WB
Purity:
Affinity purification
Target:
Biotin
Antibody Type:
Primary Antibody
Application Dilute:
WB,1:500 - 1:2000
Application Notes:
Cross-Reactivity: Species independent. Shipping: Ice Bag
Western blot analysis of recombinant Biotin-Goat-Mouse IgG Protein using Biotin Rabbit pAb (A18848) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 100 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
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