Osteopontin Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A19092
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| Article Name: | Osteopontin Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A19092 |
| Supplier Catalog Number: | A19092 |
| Alternative Catalog Number: | ABB-A19092-100UL,ABB-A19092-20UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | OPN, BNSP, BSPI, ETA-1, Osteopontin |
| The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. |
| Application Dilute: | WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Cancer,Tumor biomarkers,Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Cytoskeleton,Extracellular Matrix,Bone,Growth factors,Immunology Inflammation,Cytokines,Stem Cells,Mesenchymal Stem Cells,Cardiovascular,Angiogenesis. Shipping: Ice Bag |
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Western blot analysis of various lysates using Osteopontin Rabbit mAb (A19092) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
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