RCHY1 Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A19287
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| Article Name: | RCHY1 Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A19287 |
| Supplier Catalog Number: | A19287 |
| Alternative Catalog Number: | ABB-A19287-100UL,ABB-A19287-20UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | ZCHY, ARNIP, CHIMP, PIRH2, RNF199, ZNF363, PRO1996, RCHY1 |
| The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Cancer,Tumor suppressors,p53 pathway,Cell Biology Developmental Biology,Cell Cycle,Cell cycle inhibitors,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway. Shipping: Ice Bag |
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Western blot analysis of lysates from HCT116 cells, using RCHY1 Rabbit mAb (A19287) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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