EHMT2 Rabbit mAb, Unconjugated, Monoclonal

Article Name:	EHMT2 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A19288
Supplier Catalog Number:	A19288
Alternative Catalog Number:	ABB-A19288-20UL,ABB-A19288-100UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	G9A, BAT8, GAT8, NG36, KMT1C, C6orf30, EHMT2

This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants.

Clonality:	Monoclonal
Molecular Weight:	132 kDa
NCBI:	10919
UniProt:	Q96KQ7
Purity:	Affinity purification
Target:	EHMT2

Application Dilute:	WB,1:1000 - 1:5000\|IHC-P,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Epigenetic writers and erasers of core Histones,Cell Biology Developmental Biology,Stem Cells,Embryonic Stem Cells,Germline Stem Cells. Shipping: Ice Bag

	Western blot analysis of various lysates using EHMT2 Rabbit mAb (A19288) at 1:5000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90 s.
	Immunohistochemistry analysis of paraffin-embedded Human breast tissue using EHMT2 Rabbit mAb (A19288) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using EHMT2 Rabbit mAb (A19288) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using EHMT2 Rabbit mAb (A19288) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using EHMT2 Rabbit mAb (A19288) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using EHMT2 Rabbit mAb (A19288) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using EHMT2 Rabbit mAb (A19288) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using EHMT2 Rabbit mAb (A19288) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.