Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Alternative Names:
OCT6, OTF6, SCIP, POU3F1
Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II, positive regulation of gene expression, and positive regulation of transcription, DNA-templated. Predicted to act upstream of or within keratinocyte differentiation, nervous system development, and positive regulation of transcription by RNA polymerase II. Located in nucleoplasm.
WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:
Cross-Reactivity: Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Neuroscience, Cell Type Marker. Shipping: Ice Bag
Western blot analysis of various lysates using POU3F1 Rabbit pAb (A19330) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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