5T4 Rabbit mAb, Unconjugated, Monoclonal

Catalog Number: ABB-A19541
Article Name: 5T4 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number: ABB-A19541
Supplier Catalog Number: A19541
Alternative Catalog Number: ABB-A19541-100UL,ABB-A19541-20UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: 5T4, M6P1, 5T4AG, WAIF1
This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5 UTR results in multiple transcript variants that encode the same protein.
Clonality: Monoclonal
Molecular Weight: 46kDa
NCBI: 7162
UniProt: Q13641
Purity: Affinity purification
Target: TPBG
Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human. ResearchArea: Cancer,Tumor immunology,Tumor-associated antigens,Cell Biology Developmental Biology,Immunology Inflammation,Stem Cells. Shipping: Ice Bag
Western blot analysis of various lysates using 5T4 Rabbit mAb (A19541) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.